Low-Resolution Typing(A,B,DRB1,DQA1,DQB1)

CMDL > Low-Resolution Typing(A,B,DRB1,DQA1,DQB1)

Intended Use

To identify the Human Leucocyte antigens, DNA typing of HLA Class I (-A & -B) or Class II (-DQA1/B1 & -DRB1) alleles which is used to analyse the Histocompatibility of the recipients and donors for Transplantations
Specimen Required
  • Recipient: 1 / Purple / K2-EDTA / 2 mL
  • Donor: 1 / Purple / K2-EDTA / 2 mL
Patient Preparation

Sample collection

  • Specimen preparation: DO NOT FREEZE blood or tissue samples intended for HLA testing or crossmatching, as freezing damages the cells.
  • Storage/Transport Temperature: Samples may be stored temporarily at refrigerated temperatures (4°C to 8°C) prior to dispatch. Ensure they are packaged to maintain this temperature during transit if required by local protocols.
  • Unacceptable Conditions:
  • Remarks:
  • Stability:
Methodology

Test performed using the One Lambda LABType SSO Typing kits. LABType applies Luminex® technology to the reverse SSO DNA typing method. First, target DNA is PCR-amplified using a group-specific primer. The PCR product is biotinylated, which allows it to be detected using R-Phycoerythrin conjugated Streptavidin (SAPE). The PCR product is denatured and allowed to rehybridize to complementary DNA probes conjugated to fluorescently coded microspheres. A flow analyser, LABScan 3D identifies the fluorescent intensity of PE (phycoerythrin) on each microsphere. The assignment of the HLA typing is based on the reaction pattern compared to patterns associated with published HLA gene sequences.

Sample received to report Turnaround time (TAT)

5 Working Days

Reference Interval

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Interpretive Data

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Resources

Sample Reports

  • Enhanced Report
  • See report