Intended Use
Fever panel real-time PCR is an in-vitro nucleic acid amplification assay for the qualitative detection and differentiation of Dengue virus (DENV), Chikungunya virus (CHIKV), West Nile virus (WNV), Zika virus, Orientia tsutsugamushi (S. typhus), Rickettsia spp., Salmonella spp., Plasmodium spp., and Leptospira spp using Real-Time PCR techniques.
Specimen Required
2 Tubes / EDTA Blood / 2 mL per tube or 2 ml Plasma. (Min: 1 mL) or Urine.
Patient Preparation
- Sample Collection:
- Whole Blood: Collect in lavender (EDTA). Collection of blood before dialysis is preferred.
- Urine Specimen: Collect in a clean, nonmetal container without preservatives if the specimen is maintained refrigerated or on ice during collection and transport to the laboratory.
- Specimen Preparation: Do not freeze. Transport 4 mL whole blood in EDTA tube or 2 mL plasma. (Min: 1 mL) or urine (containers or transport tubes).
- Storage/Transport Temperature: Refrigerated
- Unacceptable Conditions: Heparinized specimens, Under- or over-filled tubes of urine container.
- Remarks: Proper aseptic technique and sample handling are critical to avoid contamination or degradation.
- Stability:
- Whole Blood/Plasma: Ambient: 24 hrs; Refrigerated: 6 days; Frozen: 6 months
- Urine: Ambient: 90 days; Refrigerated: 90 days; Frozen: Unacceptable.
Methodology
This assay uses qualitative real time PCR to detect and identify tropical fever pathogens.
Sample Received to Report Turnaround Time (TAT)
Twice in a Week (Scheduled Test)
Reference Interval
Positive or negative for presence of target tropical fever pathogen (DNA/RNA).
Interpretive Data
Refer to the report.
Resources
- Additional Technical Information
- Test Request Form
Sample Reports
- Enhanced Report
- See report