Intended Use
BK Virus Real-Time PCR Detection is in vitro nucleic acid amplification assay, based on real-time PCR technology, for the detection and quantification of BK virus (BKV) specific DNA.
Specimen Required
2 Tubes / EDTA Blood / 2 mL per tube or 2 ml Plasma. (Min: 1 mL) or Urine.
Patient Preparation
- Sample Collection:
- Whole Blood: Collect in lavender (EDTA). Collection of blood before dialysis is preferred.
- Urine Specimen: Collect in a clean, nonmetal container without preservatives if the specimen is maintained refrigerated or on ice during collection and transport to the laboratory.
- Specimen Preparation: Do not freeze. Transport 4 mL whole blood in EDTA tube or 2 mL plasma. (Min: 1 mL) or urine (containers or transport tubes) within 24 hours of collection.
- Storage/Transport Temperature: Refrigerated
- Unacceptable Conditions: Heparinized specimens, Under- or over-filled tubes of urine container.
- Remarks: Proper aseptic technique and sample handling are critical to avoid contamination or degradation.
- Stability:
- Whole Blood/Plasma: Ambient: 24 hours; Refrigerated: 6 days; Frozen: 6 months
- Urine: Ambient: 90 days; Refrigerated: 90 days; Frozen: Unacceptable.
Methodology
This test utilizes real-time PCR to detect and quantify BK virus (BKV) specific DNA in samples like human plasma or urine.
Sample received to report Turnaround time (TAT)
Twice in a Week (Scheduled Test)
Reference Interval
Positive or negative for presence of BK viral DNA.
Interpretive Data
Refer to the report.
Resources
- Additional Technical Information
- Test Request Form
Sample Reports
- Enhanced Report
- See report