B-ALL occurs predominantly in children, with 75% of all patients aged < 6 years, and is the most common leukaemia in the paediatric age group. B-ALL with ETV6::RUNX1 fusion is one of the most common subtypes of paediatric B-ALL/LBL and has a favourable prognosis. Intrachromosomal amplification of chromosome 21 (iAMP21) is a rare type of B-ALL/LBL with a poor prognosis that can show grossly abnormal chromosome 21 on karyotype analysis but is often observed by FISH while screening for ETV6::RUNX1.
- Bone Marrow: Transfer 3 mL bone marrow to a Green (Sodium Heparin). (Min: 1 mL)
- Whole Blood: Transport 5 mL whole blood. (Min: 2 mL)
- Sample collection: Non-diluted bone marrow aspirate. Collect in a sodium heparinized Vacutainer.
- Specimen preparation: Do not freeze or expose to extreme temperatures.
- Storage/Transport Temperature: Room temperature.
- Unacceptable Conditions: Frozen specimens. Clotted specimens.
- Remarks:
- Stability: Ambient: 48 hours; Refrigerated: 48 hours; Frozen: Unacceptable
Fluorescence in situ Hybridization (FISH)
Sample received to report Turnaround time (TAT)
3 working days
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Interpretive Data
Probes include: BCR-ABL1, ETV6-RUNX1, TCF3/PBX1, KMT2A(MLL), IGH, Hyperdiploidy (Chr 4, 10, 17), TP53
The most recent WHO classification of Tumours of Hematopoietic and Lymphoid Tissues (Revised 5th edition) is used for interpretation criteria for evaluation.
Resources
- Additional Technical Information
- Test Request Form
Sample Reports
- Enhanced Report
- See report