Follicular lymphoma (FL) is one of the more common lymphomas. In the fifth edition of the WHO classification of hematolymphoid tumours, we have adopted a new way of subtyping FL. The vast majority of FLs have a follicular growth pattern at least in part, are composed of centrocytes and centroblasts, and harbour the t(14;18)(q32;q21) translocation in about 85% of cases. These lymphomas are now termed classic FL and are set apart from less frequent subtypes.
Paraffin-embed tissue (3–4-micron thick sections in positively charged/silanized slides).
- Sample collection: Tumour Tissue Block
- Specimen preparation: Do not freeze or expose to extreme temperatures.
- FFPE Tissue Block: Formalin fix (10 percent neutral buffered formalin) and paraffin-embed tissue (3–4-micron thick sections in positively charged/silanized slides). Fixative duration: 6-48 hours.
- Storage/Transport Temperature: Room temperature.
- Unacceptable Conditions: Paraffin block with no tumor tissue remaining. Specimens fixed in any fixative other than 10 percent neutral buffered formalin. Decalcified specimens. Specimens with fixation delayed for more than one hour. Tissue fixed or less than 6 hours of greater than 48 hours.
- Remarks:
- Stability: Ambient: 48 hours; Refrigerated: 48 hours; Frozen: Unacceptable
Fluorescence in situ Hybridization (FISH)
Sample received to report Turnaround time (TAT)
5 working days
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Interpretive Data
Probes include: IGH-BCL2 Fusion, t(14:18), IRF4/MUM1
The most recent WHO classification of Tumours of Hematopoietic and Lymphoid Tissues (Revised 5th edition) is used for interpretation criteria for evaluation.
Resources
- Additional Technical Information
- Test Request Form
Sample Reports
- Enhanced Report
- See report