The primary genetic event in BL is the IG::MYC translocation juxtaposing MYC either to the IGH locus by the t(8;14)(q24;q32) translocation (in 80% of cases) or, less commonly, to the IGL or IGK locus by the t(8;22)(q24;q11) and t(2;8)(p12;q24) translocations, resulting in constitutive MYC expression. Demonstration of MYC breakage or IG::MYC translocation, preferably with absence of BCL2 and BCL6 translocation, may support the diagnosis of BL in the appropriate morphological and immunophenotypic context.
Paraffin-embed tissue (3–4-micron thick sections in positively charged/silanized slides).
- Sample collection: Tumour Tissue Block
- Specimen preparation: Do not freeze or expose to extreme temperatures.
- FFPE Tissue Block: Formalin fix (10 percent neutral buffered formalin) and paraffin-embed tissue (3–4-micron thick sections in positively charged/silanized slides). Fixative duration: 6-48 hours.
- Storage/Transport Temperature: Room temperature.
- Unacceptable Conditions: Paraffin block with no tumor tissue remaining. Specimens fixed in any fixative other than 10 percent neutral buffered formalin. Decalcified specimens. Specimens with fixation delayed for more than one hour. Tissue fixed or less than 6 hours of greater than 48 hours.
- Remarks:
- Stability: Ambient: 48 hours; Refrigerated: 48 hours; Frozen: Unacceptable
Fluorescence in situ Hybridization (FISH)
Sample received to report Turnaround time (TAT)
5 working days
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Interpretive Data
Probes include: MYC t(8;14), t(2:8), t(8;22)
The most recent WHO classification of Tumours of Hematopoietic and Lymphoid Tissues (Revised 5th edition) is used for interpretation criteria for evaluation.
Resources
- Additional Technical Information
- Test Request Form
Sample Reports
- Enhanced Report
- See report