Mantle cell lymphoma (MCL) is a mature B-cell neoplasm derived from the mantle zone of lymphoid follicles and typically composed of small to medium-sized monomorphic cells expressing CD5, SOX11, and cyclin D1. It is associated with CCND-family rearrangements, most commonly CCND1. If the morphology or the immunophenotype deviates from the expected features of MCL, demonstration of CCND1 rearrangement or IGH::CCND1 fusion and an extended immunohistochemistry panel is recommended, to exclude lymphomas that can express cyclin D1.
Paraffin-embed tissue (3–4-micron thick sections in positively charged/silanized slides).
- Sample collection: Tumour Tissue Block
- Specimen preparation: Do not freeze or expose to extreme temperatures.
- FFPE Tissue Block: Formalin fix (10 percent neutral buffered formalin) and paraffin-embed tissue (3–4-micron thick sections in positively charged/silanized slides). Fixative duration: 6-48 hours.
- Storage/Transport Temperature: Room temperature.
- Unacceptable Conditions: Paraffin block with no tumor tissue remaining. Specimens fixed in any fixative other than 10 percent neutral buffered formalin. Decalcified specimens. Specimens with fixation delayed for more than one hour. Tissue fixed or less than 6 hours of greater than 48 hours.
- Remarks:
- Stability: Ambient: 48 hours; Refrigerated: 48 hours; Frozen: Unacceptable
Fluorescence in situ Hybridization (FISH)
Sample received to report Turnaround time (TAT)
5 working days
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Interpretive Data
Probes include: BCL1 (CCND1) (11:14)(q13;q32)
The most recent WHO classification of Tumours of Hematopoietic and Lymphoid Tissues (Revised 5th edition) is used for interpretation criteria for evaluation.
Resources
- Additional Technical Information
- Test Request Form
Sample Reports
- Enhanced Report
- See report