CALR Exon 9 qualitative real-time PCR

CMDL > CALR Exon 9 qualitative real-time PCR

Intended Use

CALR Exon 9 qualitative real-time PCR is qualitative assay for the detection of CALR mutations in genomic DNA.

Specimen Required

Whole blood or Bone marrow in lavender (EDTA).

Patient Preparation
  • Sample Collection: 2 Tubes / EDTA Blood / 2 mL per tube or bone marrow in lavender (EDTA).
  • Specimen Preparation
    • Whole Blood: Do not freeze. Transport 5 mL whole blood. (Min: 1 mL)
    • Bone Marrow: Transport 3 mL bone marrow. (Min: 1 mL)
  • Storage/Transport Temperature: Refrigerated
  • Unacceptable Conditions
    • Plasma, serum, FFPE tissue blocks/slides, or frozen tissue.
    • Specimens collected in anticoagulants other than EDTA.
    • Clotted or grossly haemolyzed specimens.
  • Remarks: –
  • Stability:
    • Whole Blood: Ambient: 24 hrs; Refrigerated: 6 days; Frozen: 6 months
    • Bone Marrow: Ambient: 1 week; Refrigerated: 1 week; Frozen: 1 week
Methodology

This test uses real-time qualitative assay for detection of somatic mutations in the region c.1091_1162 of exon 9 in the CALR gene and allows the identification of the two major CALR mutations.

Sample Received to Report Turnaround Time (TAT)

Twice in a week (Scheduled Test)

Reference Interval

–

Interpretive Data

Refer to report.

Resources

  • Additional Technical Information
  • Test Request Form

Sample Reports

  • Enhanced Report
  • See report