Intended Use
To qualitatively detect Chikungunya virus RNA using one-step real-time reverse transcription PCR (RT-PCR) for diagnosing Chikungunya infection.
Specimen Required
2 Tubes / EDTA Blood / 2 mL per tube or 2 ml Plasma. (Min: 1 mL)
Patient Preparation
- Sample collection: Collect in lavender (EDTA). Collection of blood before dialysis is preferred.
- Specimen preparation: Do not freeze. Transport 4 mL whole blood in EDTA tube or 2 mL plasma. (Min: 1 mL)
- Storage/Transport Temperature: Refrigerated
- Unacceptable Conditions: Heparinized specimens.
- Remarks: Proper aseptic technique and sample handling are critical to avoid contamination or degradation.
- Stability: Ambient: 24 hours; Refrigerated: 5 days; Frozen: 6 months
Methodology
The test uses one-step real-time RT-PCR, where viral RNA is reverse-transcribed to cDNA followed by PCR amplification with specific primers and probes targeting a conserved region of the Chikungunya genome.
Sample received to report Turnaround time (TAT)
Twice in a Week (Scheduled Test)
Reference Interval
Positive or negative for presence of Chikungunya RNA.
Interpretive Data
Refer to the Report.
Resources
- Additional Technical Information
- Test Request Form
Sample Reports
- Enhanced Report
- See report