Intended Use
To qualitatively detect the presence of Clostridium difficile specific toxin genes (Toxin A, Toxin B, binary toxins cdtA and cdtB) using real-time PCR.
Specimen Required
Soft or Liquid Stool.
Patient Preparation
- Sample Collection: Collect stool or rectal swab samples aseptically.
- Specimen Preparation: Preserve stool sample in Cary-Blair transport medium.
- Storage/Transport Temperature: Refrigerated
- Unacceptable Conditions: Specimens in media or preservatives.
- Remarks: Proper aseptic technique and sample handling are critical to avoid contamination or degradation.
- Stability: Ambient: 48 hours; Refrigerated: 5 days; Frozen: 1 week
Methodology
The test employs real-time PCR for detection and identification of different toxin genes of Clostridium difficile.
Sample Received to Report Turnaround Time (TAT)
Twice in a week (Scheduled Test)
Reference Interval
Positive or negative for presence of C. difficile toxin genes.
Interpretive Data
Refer to the Report.
Resources
- Additional Technical Information
- Test Request Form
Sample Reports
- Enhanced Report
- See report