HLA Class I & II Antibody Screen(Luminex)

The test is performed using the One Lambda LABScreenTM Mixed Class I & II assays for the detection of HLA Class I, HLA Class II, and MICA antibodies in the patient’s serum/plasma. The principle of the assay involves incubating the test serum with LABScreen beads which are coated with HLA Class I/ Class II and MICA antigens. Any HLA antibodies present in the test serum bind to the antigens on the beads and are then labeled with R-Phycoerythrin (PE)-conjugated goat anti-human IgG. The Luminex LabScan 100, LABScan 3D, or FLEXMAP 3D flow analyzer is used for analysis. This instrument simultaneously detects the fluorescent emission of PE and a dye signature from each bead, allowing for near real-time data acquisition. The positive/negative cut-off is determined by selecting an NBG ratio that gives a significant shift over the background fluorescent value, validated using negative control serum and reference allosera with defined HLA antibody specificity. For LABScreen Mixed, if any one bead in the mixed assay is positive, then the result should be assigned as positive. The LABScreenTM Mixed assay specifically detects the presence of antibodies to Class I and/or Class II HLA antigens and MICA antigens.