Human Adenovirus qualitative real-time PCR, Plasma

Intended Use

To qualitatively detect human adenovirus DNA using real-time PCR for diagnosing adenoviral infections.

Specimen Required

2 Tubes / EDTA Blood / 2 mL per tube or 2 ml Plasma. (Min: 1 mL)

Patient Preparation

Sample Collection: Collect the blood in lavender (EDTA). Collection of blood before dialysis is preferred.
Specimen Preparation: Do not freeze. Transport 4 mL whole blood in EDTA tube or 2 mL plasma. (Min: 1 mL)
Storage/Transport Temperature: Refrigerated
Unacceptable Conditions: Heparinized specimens
Remarks: Proper aseptic technique and sample handling are critical to avoid contamination or degradation.
Stability: Ambient: 24 hrs; Refrigerated: 6 days; Frozen: 6 months

Methodology

The test involves real-time PCR amplification of human adenovirus DNA using multiplexed fluorescent probes in a single tube assay.

Sample Received to Report Turnaround Time (TAT)

Twice in a Week (Scheduled Test)

Reference Interval

Positive or negative for presence of Human Adenovirus DNA.

Interpretive Data

Refer to the Report.

Resources