The main feature of LBCL-IRF4-R is a chromosomal translocation of IRF4 (6p25.3), most frequently with the IGH locus, or rarely with IGK or IGL. A lack of this alteration should lead to the consideration of various differential diagnoses, although in rare cases IRF4 rearrangement might be cryptic to molecular cytogenetic methods. BCL2 and MYC are virtually never rearranged, whereas chromosomal breaks affecting the BCL6 locus have been reported.
Paraffin-embed tissue (3–4-micron thick sections in positively charged/silanized slides).
- Sample collection: Tumour Tissue Block
- Specimen preparation: Do not freeze or expose to extreme temperatures.
- FFPE Tissue Block: Formalin fix (10 percent neutral buffered formalin) and paraffin-embed tissue (3–4-micron thick sections in positively charged/silanized slides). Fixative duration: 6-48 hours.
- Storage/Transport Temperature: Room temperature.
- Unacceptable Conditions: Paraffin block with no tumor tissue remaining. Specimens fixed in any fixative other than 10 percent neutral buffered formalin. Decalcified specimens. Specimens with fixation delayed for more than one hour. Tissue fixed or less than 6 hours of greater than 48 hours.
- Remarks:
- Stability: Ambient: 48 hours; Refrigerated: 48 hours; Frozen: Unacceptable
Fluorescence in situ Hybridization (FISH)
Sample received to report Turnaround time (TAT)
5 working days
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Interpretive Data
Probes include: IRF4/DUSP22 (6p25) Gene Rearrangement
The most recent WHO classification of Tumours of Hematopoietic and Lymphoid Tissues (Revised 5th edition) is used for interpretation criteria for evaluation.
Resources
- Additional Technical Information
- Test Request Form
Sample Reports
- Enhanced Report
- See report