Intended Use
Burkitt lymphoma (BL) is a rare, but fast-growing type of non-Hodgkin lymphoma (NHL). The translocation between the MYC gene locus at 8q24 and the immunoglobulin genes (IG) for the kappa light chain at 2p12 (IGK), for the heavy chain at 14q32 (IGH) or for the lambda light chain at 22q11 (IGL), juxtapose the MYC gene to an IG enhancer, resulting in overexpression of MYC. About 80% of BL patients show the MYC rearrangement t(8;14)(q24;q32) while approximately 10% show a translocation between the MYC gene region and IGK or IGL. Additional breakpoints in BCL2, BCL6 and CCND1 are indicators for an aggressive course and short overall survival.
Patient Preparation
- Sample collection: Non-diluted bone marrow aspirate. Collect in a sodium heparinized Vacutainer.
- Specimen preparation: Do not freeze or expose to extreme temperatures.
- Bone Marrow: Transfer 3 mL bone marrow to a Green (Sodium Heparin). (Min: 1 mL)
- Whole Blood: Transport 5 mL whole blood. (Min: 2 mL)
- Storage/Transport Temperature: Room temperature.
- Unacceptable Conditions: Frozen specimens. Clotted specimens.
- Remarks:
- Stability: Ambient: 48 hours; Refrigerated: 48 hours; Frozen: Unacceptable
Methodology
Fluorescence in situ Hybridization (FISH)
Sample received to report Turnaround time (TAT)
3 working days
Reference Interval
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Interpretive Data
The most recent WHO classification of Tumours of Hematopoietic and Lymphoid Tissues (Revised 5th edition) is used for interpretation criteria for evaluation.
Resources
- Additional Technical Information
- Test Request Form
Sample Reports
- Enhanced Report
- See report