MYD88 L265P qualitative real-time PCR

Intended Use

To qualitatively detect the L265P mutation in c.794T>C in myeloid differentiation factor 88 (MYD88) gene.

Specimen Required

Whole blood or Bone marrow in lavender (EDTA) or FFPE tumor tissue.

Patient Preparation

Sample Collection: 2 Tubes / EDTA Blood / 2 mL per tube or bone marrow in lavender (EDTA).
Specimen Preparation
- Whole Blood: Do not freeze. Transport 5 mL whole blood. (Min: 1 mL)
- Bone Marrow: Transport 3 mL bone marrow. (Min: 1 mL)
- FFPE Tumor Tissue: Formalin-fixed (10 percent neutral buffered formalin) and paraffin-embedded tissue. Protect from excessive heat.

Storage/Transport Temperature
- Whole Blood, Bone Marrow: Refrigerated
- FFPE Tumor Tissue: Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.

Unacceptable Conditions
- Plasma, serum. Specimens collected in anticoagulants other than EDTA.
- Clotted or grossly haemolyzed specimens.
- FFPE Tumor Tissue: Specimens fixed in any fixative other than 10 percent neutral buffered formalin.
- Bone specimens submitted in non-EDTA decalcifier.

Remarks: If multiple specimens (blocks or slides) are sent to CMDL, they must be accompanied by one of the following: an order comment indicating that the CMDL pathologist should choose the specimen most appropriate for testing (e.g., “Choose best block”), or individual orders for each sample submitted. If multiple specimens are sent to CMDL without a request for pathologist block/slide selection or individual orders, they will be held until clarification is provided.

Stability
- Whole Blood or Bone Marrow: Refrigerated: 7 days; Frozen: Unacceptable
- FFPE Tumor Tissue: Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable

Methodology

This test uses real-time qualitative multiplex PCR reaction to detect and identify the MYD88 mutation.

Sample Received to Report Turnaround Time (TAT)

Twice in a week (Scheduled Test)

Reference Interval

–

Interpretive Data

Refer to report.

Resources