Parvovirus B19 quantitative real-time PCR

Intended Use

To quantitatively detect and measure Human Parvovirus B19 DNA using real time PCR.

Specimen Required

2 Tubes / EDTA Blood / 2 mL per tube or 2 ml Plasma. (Min: 1 mL)

Patient Preparation

Sample Collection: Collect the blood in lavender (EDTA). Collection of blood before dialysis is preferred.
Specimen Preparation: Do not freeze. Transport 4 mL whole blood in EDTA tube or 2 mL plasma. (Min: 1 mL)
Storage/Transport Temperature: Refrigerated
Unacceptable Conditions: Heparinized specimens
Remarks: Proper aseptic technique and sample handling are critical to avoid contamination or degradation.
Stability: Ambient: 24 hrs; Refrigerated: 6 days; Frozen: 6 months

Methodology

This test uses real-time PCR to amplify and quantitatively detect using specific primers and probes targeting Parvovirus B19 DNA.

Sample Received to Report Turnaround Time (TAT)

Twice in a Week (Scheduled Test)

Reference Interval

Positive or negative for presence of Parvovirus B19 DNA.

Interpretive Data

Refer to the Report.

Resources