Intended Use
t(8;21)(q21;q22) is the most common translocation in de novo AML occurring in up to 20% of adult and 40% of pediatric cases. The translocation fuses RUNX1 with RUNX1T1 to produce a RUNX1/RUNX1T1 fusion gene located on the derivative chromosome 8. For these patients, the prognosis after intensive chemotherapy is better than for the majority of AML patients. Small hidden interstitial insertions resulting in a RUNX1/RUNX1T1 rearrangement have been found, necessitating the use of a breakpoint spanning rather than a breakpoint flanking FISH probe.
Patient Preparation
- Sample collection: Non-diluted bone marrow aspirate. Collect in a sodium heparinized Vacutainer.
- Specimen preparation: Do not freeze or expose to extreme temperatures.
- Bone Marrow: Transfer 3 mL bone marrow to a Green (Sodium Heparin). (Min: 1 mL)
- Whole Blood: Transport 5 mL whole blood. (Min: 2 mL)
- Storage/Transport Temperature: Room temperature.
- Unacceptable Conditions: Frozen specimens. Clotted specimens.
- Remarks:
- Stability: Ambient: 48 hours; Refrigerated: 48 hours; Frozen: Unacceptable
Methodology
Fluorescence in situ Hybridization (FISH)
Sample received to report Turnaround time (TAT)
3 working days
Reference Interval
————-
Interpretive Data
The most recent WHO classification of Tumours of Hematopoietic and Lymphoid Tissues (Revised 5th edition) is used for interpretation criteria for evaluation.
Resources
- Additional Technical Information
- Test Request Form
Sample Reports
- Enhanced Report
- See report