PML-RARA Translocation by FISH
I. Ordering Recommendation: Rapid confirmatory diagnostic test for APL.
II. Specimen Required:
A. Collect: Non-diluted bone marrow aspirate. Collect in a heparinized syringe.
B. Specimen Preparation: Do not freeze or expose to extreme temperatures. Transfer 3 mL bone marrow to a green (sodium heparin). (Min: 0.5 mL)
C. Storage/Transport Temperature: Room temperature.
D. Unacceptable Conditions: Frozen specimens. Clotted specimens.
E. Remarks:
F. Stability: Ambient: 48 hours; Refrigerated: 48 hours; Frozen: Unacceptable
III. Methodology: Fluorescence in situ Hybridization (FISH)
IV. Performed:
V. Reported (TAT):
VI. Interpretive Data:
Resources
Sample Reports
Note:This test must be ordered using the Oncology test request form.